Site-specific protein immobilization by fusion to self-labeling tags.
Download scientific diagram | Site-specific protein immobilization by fusion to self-labeling tags. (a) The SNAP-tag, 20 kDa in size, reacts with O 6 -benzylguanine. (b) The CLIP-tag (also 20 kDa) reacts with O 2 -benzylcytosine substrates, whereas the HaloTag (33 kDa) forms a covalent bond through nucleophilic displacement of halides from alkyl halide substrates. from publication: Recent advances in covalent, site-specific protein immobilization | The properties of biosensors, biomedical implants, and other materials based on immobilized proteins greatly depend on the method employed to couple the protein molecules to their solid support. Covalent, site-specific immobilization strategies are robust and can provide the | Immobilized Proteins, Protein Chemistry and Applied Microbiology | ResearchGate, the professional network for scientists.
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A preparation strategy for protein-oriented immobilized silica magnetic beads with Spy chemistry for ligand fishing - ScienceDirect
PDF) Recent advances in covalent, site-specific protein immobilization
Scheme of the two step bioconjugation of QDs using SNAP tag fusion
Frontiers Detecting and measuring of GPCR signaling – comparison of human induced pluripotent stem cells and immortal cell lines
Site-specific protein immobilization by fusion to self-labeling tags.
Recent advances in covalent, site-specific
PDF) Recent advances in covalent, site-specific protein immobilization
Visualizing and Manipulating Biological Processes by Using HaloTag and SNAP‐ Tag Technologies - Hoelzel - 2020 - ChemBioChem - Wiley Online Library
Protein Labeling and Crosslinking by Covalent Aptamers - Tivon - 2021 - Angewandte Chemie International Edition - Wiley Online Library
Putting precision and elegance in enzyme immobilisation with bio-orthogonal chemistry - Chemical Society Reviews (RSC Publishing) DOI:10.1039/D1CS01004B
Enzyme‐mediated methodologies for protein modification and bioconjugate synthesis - Matsumoto - 2012 - Biotechnology Journal - Wiley Online Library
PDF] Site-Specific Labeling of Proteins Using Unnatural Amino Acids
